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Vigor series serum-free medium for insect cells

Serum-free media specially developed for efficient large-scale culture of insect cells

BioEngine team started to research techniques for the culture of insect cells (Sf9, High five) in 1995 and successfully developed the first serum-free medium for insect cells in 1998. Based on more than 20 years of technology accumulation and an AI high-throughput data screening platform, BioEngine now releases the latest Vigor series serum-free media for insect cells to meet customers' demand for high-efficient large-scale culture of insect cells. The Vigor series serum-free media for insect cells have been applied in large-scale manufacturing of a variety of products including COVID-19 vaccines and VLPs.

Vigor series serum-free medium for insect cells

Features

Serum-free

Animal-derived component-free

Protein-free

Available for high-density culture of insect cells

Available for high protein expression in insect cells

Available in powder and liquid format

Suitable for both Sf9 and High five cells

Advantages

Animal-derived component-free; TSE/BSE statement available on demand;

Distinctive culture results proven in numerous studies;

Optional powder media for use in large-scale manufacturing with easy preparation procedures;

Powder media capable of a single batch size of 60,000 L;

Excellent inter-batch consistency (CPK*>1.33);

Full traceability by EU-certified ISO13485:2016 Quality Management System;

Complete clinical application supporting documents can be provided. *CPK: Process Capability Index. CPK>1.33 indicates good process control and small inter-batch difference of products.

Order Information
Basal Medium
Application
Product Name
Cat. No.
Size
Form
Product Instruction (pdf)Inquiries /Sample application
Suitable for both Sf9 and High five cells
Support the production of subunit vaccine such as Porcine circovirus, Classical Swine Fever virus, and Porcine parvovirus or VLP
Vigor-S100 Insect Cell Serum-free Medium
EXP0101102
1 L Liquid Inquiries /Sample application
Vigor-S100S Insect Cell Serum-free Medium
EXP0109401
100 L Powder
EXP0109402
5 L Powder
Vigor-S100S Additive
EXP0109501
100 ml Liquid
EXP0109502
5 ml Liquid
Suitable for both Sf9 and High five cells
Support the production of subunit vaccines such as COVID-19, influenza and other VLPs
Vigor-S101 Insect Cell Serum-free Medium EXP0102201 1 L Liquid
Vigor-S101S Insect Cell Serum-free Medium EXP0107401 100 L Powder
EXP0107403 5 L Powder
Vigor-S101S Additive EXP0107501 100 ml Liquid
EXP0107503 5 ml Liquid
*DrivingM® is a registered trademark of Shanghai BioEngine Sci-Tech Co., Ltd.
   The powder culture medium is used in combination with the corresponding 1000 × additives.
Performance
Vaccines

Inoculated with 1×106 cells/ml and passaged every 48 h in Vigor-S101S, Sf9 cell density reached 3-5×106 cells/ml (doubling time of 21-30 h), and High five cell density reached 5-7×106 cells/ml (doubling time of 17-21 h). Indicating Vigor-S101S allows for long-term stable passage.

 

Sf9 cells in Vigor-S101S reached a maximum density of 12-14×106 cells/ml, with a faster growth rate, higher density, and longer maintenance time than in a global brand medium.
The density of High five cells in Vigor-S101S reached a maximum of 9-11×106 cells/ml, which was consistent with the level in a medium of a well-known brand dedicated to High five culture.

 

Vigor-S101S outperformed similar products on the market in terms of titer when using Sf9 cells to produce baculovirus.

Vigor-S101S showed advantages in expressing protein yield using High five cells..

Let us contact you

We have a professional technical team to provide high quality one-stop cell culture service from process development
and optimization, medium formulation design to medium processing and manufacturing for all biopharmaceutical companies.

If you have any question, please click "Quick Message" and leave your message,  we will reply to you as soon as possible.
If anything urgent, please call (86)21-68582660-2792.

BioEngine

3F&4F, Building 3, Lane 396, Lvzhou Ring Road, Minhang District, Shanghai, PRC

(86)21-68582660-2792

globalsales@bio-engine.com.cn

Work hours:9:00-17:00