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Pesche series serum-free medium for Vero cells

CD medium specially developed for efficient culture of Vero cells for virus production

Pesche Vero CD Media is designed for the growth of adherent Vero cells for virus production, supporting its high-density expansion, continuous passaging culture, and other virus production modes without serum supplemented. It meets the nutritional requirements of cells in different states of virus production and promotes cell proliferation and virus yield. The serum-free, animal-derived component-free, and ultra-low protein formulation facilitates downstream purification and maximizes the safety and reliability of the production process. BioEngine can tailor the composition of the medium and provide customized formulations to meet the production requirements of different viruses. Pesche-CD001S supports the large-scale production of COVID-19 vaccines, human rabies vaccines, and rotavirus vaccines, and has successfully supported the human rabies vaccine program into phase III clinical trial.

Pesche series serum-free medium for Vero cells

Features

Serum-free

Animal-derived component-free

Ultra-low protein

Chemical Defined

Supporting high density growth of Vero cells

Available in powder and liquid format

Advantages

Animal-derived component-free; TSE/BSE statement available on demand;

The culture effect is comparable to that of MEM with 10% serum;

High seeding ratio, short doubling time, high cell density, simple and convenient passaging operation.

Supporting continuous passages with no significant attenuation of cells in high generations;

Suitable for culture in microcarrier reactors, reducing production costs and improving production efficiency;

Full traceability by EU-certified ISO13485:2016 Quality Management System;

Complete documents in support of CTA for easier regulatory submission.

Order Information
Basal Medium
Application
Product Name
Cat. No.
Size
Form
Product Instruction (pdf)Inquiries /Sample application

Vero cells

Support the vaccine production of new COVID-19, rabies, rotavirus, etc.
Pesche-CD001S Vero CD Medium EXP0113101 200 L
Powder+Additive


Inquiries /Sample application
EXP0113102 100 L
Powder+Additive
EXP0113103 10 L
Powder+Additive
Pesche-CD001 Vero CD Medium
EXP0113104
1 L
Liquid+Additive
Performance
Vaccines

Cell growth

Vero cells cultured with Pesche-CD001S showed a clear contour, good morphology, and vigorous growth, allowing a high seeding the ratio for cell passaging.

 

Cell passaging

Vero cells were cultured for three consecutive passages in Pesche-CD001S, similar serum-free medium (Brand A), and MEM (10% serum) medium to fully acclimate the cells. Cells were detached into suspensions according to the cell passaging procedure, and T25 cell culture flasks were inoculated at a density of 2×104 cells/cm2 and sampled and counted every 24 hours to obtain cell growth curves for the three media, as shown in the figure below.

 

Virus production

Rabies viruses were produced in Pesche-CD001S and were collected at 48-312 h, all above the potency of 105.0 LD50/ml to meet the production requirements.

Let us contact you

We have a professional technical team to provide high quality one-stop cell culture service from process development
and optimization, medium formulation design to medium processing and manufacturing for all biopharmaceutical companies.

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If anything urgent, please call (86)21-68582660-2792.

BioEngine

3F&4F, Building 3, Lane 396, Lvzhou Ring Road, Minhang District, Shanghai, PRC

(86)21-68582660-2792

globalsales@bio-engine.com.cn

Work hours:9:00-17:00