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ACgro CD Media for Adherent Cells

Serum-Free Culture · Stable for Vaccine Production · High Virus Yield

Traditionally, adherent cells are cultured using culture medium supplemented with 10% serum. However, serum poses challenges such as significant batch-to-batch variability, animal-derived risks, and uncontrolled costs. ACgro CD Medium is a chemically defined, animal component-free solution that overcomes serum dependency. It supports the growth of various adherent cells, including Vero, 293, and MDCK, providing a safer, more stable, and cost-effective cell culture solution for vaccine development, production, and basic research.

ACgro CD Medium for Adherent Cell

Features

Serum-free, chemically defined, animal-derived component free, ultra-low protein

Supports serum-free culture for Vero, MDCK, DF-1, 293, and other adherent cell lines.

Scalable in cell factories, microcarriers & multilayer flasks

Ideal for the production of various human and veterinary vaccines, including rabies, hand-foot-and-mouth disease (HFMD), herpes zoster, varicella, and more.

Advantages

Safe & Stable

  • Ensuring reliable vaccine production
  • Chemically defined formulation Eliminates serum-induced batch variability
  • Maintains morphology & >90% viability beyond Passage 50

Cost-Effective & Controllable

  • Eliminates serum price volatility
  • Reduces QC costs through serum-free testing
  • Minimizes contamination risks and production losses

Regulatory compliance

  • GMP-compliance facility with ISO 13485 & MDSAP (FDA) certified
  • Complete regulatory support documentation

Custom Solutions

  • Custom-optimized for specific project requirements
  • 30+ years dedicated R&D expertise
  • Proven in 100+ vaccine development programs
  • End-to-end technical service support
Order Information
Basal Medium
Product Name
Cat. No.
Form
Size
Packaging
Note
ACgro CD Medium TP0112901E Powder 10 L Bag
  • Serum-free, chemically defined, animal-derived component free, ultra-low protein
  • Add additive to poeder media at 0.75‰ (v/v)
TP0112903E Powder 200 L Bag
ACgro Additive
 
TP0202201 Liquid 50 mL Bottle
TP0202202
Liquid
150 mL
Bottle
Performance
Vaccines

1. Optimal morphology

Vero cells cultured in ACgro CD medium exhibited clear, intact boundaries and typical spindle-shaped morphology, comparable to both traditional 10% serum-containing cultures and serum-free media of Brand A and Brand B.

 

2. Long-term stable passaging

ACgro CD medium supports consistent long-term subculture of Vero cells for extended passages (≥80 generations) with stable growth characteristics.

 

3. Shorter doubling time

Vero cells cultured in ACgro CD medium exhibited a doubling time of 23 hours — comparable to serum-containing cultures (MEM+10% FBS) and significantly shorter than other commercial serum-free media.

 

4.Significantly Enhanced PEDV Yield

PEDV (Porcine Epidemic Diarrhea Virus) titers in ACgro CD medium reached 10⁸.¹¹TCID₅₀/mL — a significant increase over both traditional culture (MEM+10% FBS) and other commercial serum-free media.

 

 

5.Significantly Enhanced PRV Yield

In ACgro CD medium, the pseudorabies virus (PRV) titer reached 108.5 TCID₅₀/mL, comparable to traditional serum-containing culture (DMEM + 2% FBS) and significantly higher than other commercial serum-free media.

 

Documents

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and optimization, medium formulation design to medium processing and manufacturing for all biopharmaceutical companies.

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(86)21-68582660-2792

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